The knowledge transfer among the members of the virtual enterprise can increase profits and the incentive behavior of chief enterprises can appropriately decrease the risk and cost of the federate enterprise’s knowledge transfer,and improve the entire profits of the incentive behavior. According to the evolutionary game theory and the basic theory of knowledge transfer, we apply the entire simulation modeling method for multiagent complex adaptive systems to construct the simulation modeling of knowledge transfer incentive behavior on the NetLogo simulation platform. The strategy which is taken during the knowledge transfer incentive behavior by analyzing the the knowledge transfer incentive behavior of the chief enterprise and the evolutionary game of the members’ knowledge transfer under different return parameters. By analyzing the evolutionary game of the virtual enterprises’ knowledge transfer incentive mechanism will contribute to the construction of the virtual enterprises’ knowledge transfer incentive mechanism.