Aiming at optimization problems of management of explosive storage, an optimized online operation method of explosives storage based on the Radio Frequency IDentification (RFID) technology and genetic algorithm is proposed, making management of explosives storage more efficient, informatization, secure, intelligent. The information of explosives warehouse are acquired by RFID realtime technology, and an assignment strategy for location of explosive warehouse is proposed. The mathematical model of explosives storage optimization is constructed through analyzing operation characteristics of explosives storage and requirements, and the complicated model is solved by the genetic algorithm. The simulation results show that the proposed method can improve utilization rate of warehouse space, and optimize the walking path in the process of taking and putting the explosives, as well as solve the operating problems under the constraint condition, such as a validity of explosives.